Please click here to request reagents from the Pfaff Laboratory.

• 0.5 M EDTA pH 8.0
  
  
• Ampicillian Stock
  
  
• Chick Embryo Electroportion
  
  
• Fixing Embryos
  
  
• Immunofluorescent Staining of Sectioned Embryos
  
  
• In Vitro Double Immunoprecipitation
  
  
• Luria Broth (25g/L)
  
  
• Luria Broth Agar 1.5%
  
  
• Preparation of ES cells for Blastocyst Injection
  
  
• Preparation of PMEFs
  
  
• “Quick” Embryo Genotyping Protocol
  
  
• Tail Lysis Buffer
  
  
• Tissue Preparation for In Situ Hybridization and Immunohistochemistry
  
  
• Updated In Situ Protocol
  
  
• Whole Mount X-Gal Staining
  
  
• ES to Motor Neurons
  
  
• Feeder Preparation
  
  

• 3M NaOAc pH 5.2
  
  
• 5M NaCl
  
  
• 0.2M PB pH 7.2
  
  
• 10% SDS
  
  
• 20X SSC
  
  
• 50 x TAE
  
  
• TE
  
  
• 1M Tris pH 7.2
  
  
• Denaturing Solution
  
  
• Neutralizing Solution
  
  
• 1% Fast Green
  
  
• KGB Buffer
  
  
• 30% Sucrose
  
  
• Artificial CSF for Dextran Fills
  
  
• 20 X SSPE
  
  
• 10X PBS