| Probe |
Ex (nm) |
Em (nm) |
MW |
Notes |
| Reactive and conjugated probes |
| Hydroxycoumarin |
325 |
386 |
331 |
Succinimidyl ester |
| Aminocoumarin |
350 |
445 |
330 |
Succinimidyl ester |
| Methoxycoumarin |
360 |
410 |
317 |
Succinimidyl ester |
| Cascade Blue |
375;400 |
423 |
596 |
Hydrazide |
| Lucifer yellow |
425 |
528 |
|
|
| NBD |
466 |
539 |
294 |
NBD-X |
| R-Phycoerythrin (PE) |
480;565 |
578 |
240 k |
|
| PE-Cy5 conjugates |
480;565;650 |
670 |
|
aka Cychrome, R670, Tri-Color, Quantum Red |
| PE-Cy7 conjugates |
480;565;743 |
767 |
|
|
| APC-Cy7 conjugates |
650;755 |
767 |
|
PharRed |
| Red 613 |
480;565 |
613 |
|
PE-Texas Red |
| Fluorescein |
495 |
519 |
389 |
FITC; pH sensitive |
| FluorX |
494 |
520 |
587 |
(AP Biotech) |
| BODIPY-FL |
503 |
512 |
|
|
| TRITC |
547 |
572 |
444 |
TRITC |
| X-Rhodamine |
570 |
576 |
548 |
XRITC |
| Lissamine Rhodamine B |
570 |
590 |
|
|
| PerCP |
490 |
675 |
|
Peridinin chlorphyll protein |
| Texas Red |
589 |
615 |
625 |
Sulfonyl chloride |
| Allophycocyanin (APC) |
650 |
660 |
104 k |
|
| TruRed |
490,675 |
695 |
|
PerCP-Cy5.5 conjugate |
| Alexa Fluor dyes (Molecular Probes) |
| Alexa Fluor 350 |
346 |
445 |
410 |
|
| Alexa Fluor 430 |
430 |
545 |
701 |
|
| Alexa Fluor 488 |
494 |
517 |
643 |
|
| Alexa Fluor 532 |
530 |
555 |
724 |
|
| Alexa Fluor 546 |
556 |
573 |
1079 |
|
| Alexa Fluor 555 |
556 |
573 |
1250 |
|
| Alexa Fluor 568 |
578 |
603 |
792 |
|
| Alexa Fluor 594 |
590 |
617 |
820 |
|
| Alexa Fluor 633 |
621 |
639 |
1200 |
|
| Alexa Fluor 647 |
650 |
668 |
1250 |
|
| Alexa Fluor 660 |
663 |
690 |
1100 |
|
| Alexa Fluor 680 |
679 |
702 |
1150 |
|
| Alexa Fluor 700 |
696 |
719 |
|
|
| Alexa Fluor 750 |
752 |
779 |
|
|
| Cy Dyes (AP Biotech) |
| Cy2 |
489 |
506 |
714 |
|
| Cy3 |
(512);550 |
570;(615) |
767 |
|
| Cy3.5 |
581 |
596;(640) |
1102 |
|
| Cy5 |
(625);650 |
670 |
792 |
|
| Cy5.5 |
675 |
694 |
1128 |
|
| Cy7 |
743 |
767 |
818 |
|
| Nucleic acid probes |
| Hoechst 33342 |
343 |
483 |
616 |
AT-selective |
| DAPI |
345 |
455 |
|
AT-selective |
| Hoechst 33258 |
345 |
478 |
624 |
AT-selective |
| SYTOX Blue |
431 |
480 |
~400 |
DNA |
| Chromomycin A3 |
445 |
575 |
|
CG-selective |
| Mithramycin |
445 |
575 |
|
|
| YOYO-1 |
491 |
509 |
1271 |
|
| SYTOX Green |
504 |
523 |
~600 |
DNA |
| SYTOX Orange |
547 |
570 |
~500 |
DNA |
| Ethidium Bromide |
493 |
620 |
394 |
|
| 7-AAD |
546 |
647 |
|
7-aminoactinomycin D, CG-selective |
| Acridine Orange |
503 |
530/640 |
|
DNA/RNA |
| TOTO-1, TO-PRO-1 |
509 |
533 |
|
Vital stain, TOTO: Cyanine Dimer
TO-PRO: Cyanine Monomer |
| Thiazole Orange |
510 |
530 |
|
|
| Propidium Iodide (PI) |
536 |
617 |
668.4 |
|
| TOTO-3, TO-PRO-3 |
642 |
661 |
|
|
| LDS 751 |
543;590 |
712;607 |
472 |
DNA (543ex/712em), RNA (590ex/607em) |
| Cell function probes |
| Indo-1 |
361/330 |
490/405 |
1010 |
AM ester. Low/High Ca++, |
| Fluo-3 |
506 |
526 |
855 |
AM ester. pH > 6 |
| DCFH |
505 |
535 |
529 |
2'7'Dichorodihydrofluorescein, oxidized form |
| DHR |
505 |
534 |
346 |
Dihydrorhodamine 123, oxidized form,
light catalyzes oxidation |
| SNARF |
548/579 |
587/635 |
|
pH 6/9 |
| Fluorescent Proteins |
| Y66F |
360 |
508 |
|
|
| Y66H |
360 |
442 |
|
|
| EBFP |
380 |
440 |
|
(Clontech) Quantum yield 0.18 |
| Wild Type |
396,475 |
508 |
26k |
Quantum yield 0.77 |
| GFPuv |
385 |
508 |
|
(Clontech) |
| ECFP |
434 |
477 |
|
(Clontech) Quantum yield 0.40 |
| AmCyan1 |
458 |
489 |
|
(Clontech) Quantum yield 0.24; Tetramer |
| Y66W |
436 |
485 |
|
|
| S65A |
471 |
504 |
|
|
| S65C |
479 |
507 |
|
|
| S65L |
484 |
510 |
|
|
| S65T |
488 |
511 |
|
|
| EGFP |
489 |
508 |
26k |
(Clontech) Quantum yield ~0.60 |
| ZsGreen1 |
493 |
505 |
105k |
(Clontech) Quantum yield 0.91?; Tetramer |
| EYFP |
514 |
527 |
26k |
(Clontech) Quantum yield 0.61 |
| ZsYellow1 |
527 |
539 |
|
(Clontech) Tetramer |
| DsRed, DsRed2 (RFP) |
558 |
583 |
~110k |
(Clontech) Quantum yield 0.79. Tetramer. |
| DsRed monomer |
556 |
586 |
~28k |
(Clontech) |
| AsRed2 |
576 |
592 |
~110k |
(Clontech) Tetramer |
| mRFP1 |
584 |
607 |
~30k |
(Tsien lab) Quantum yield 0.25 |
| HcRed1 |
588 |
618 |
~52k |
(Clontech) Dimer |
| Other probes |
| Monochlorobimane |
380 |
461 |
226 |
Glutathione probe |
| Calcein |
496 |
517 |
623 |
pH > 5 |
